Posed as specific functional nano-compartment of plasma membrane, porosome represents highly active nano-machinery, responsible for transmembrane secretion of exporting molecules.  Porosome comprises membrane-associated proteins and lipids assembled into not random 3D nano-assembly, revealing constant nano-architecture, regular sizes, form, chemical content and distribution pattern on the apical membrane of glandular cells and/or presynaptic surface. Up to date the spatial nanoarchitecture of porosome is well documented at nano-scale, due to broadly used atomic force microscopic, electron microscopic and other high-resolution technologies. However, much less is known about structural, chemical, functional and quantitative fluctuations of porosome depending on functional state of given cell/tissue type, genetic/epigenetic control mechanisms, pharmacological and toxicological treatment as well as pathological conditions. The proposed innovative concept in neuroscience represents interdisciplinary, multimodal and multi-scale project addressed to hypothesis, that propionic acid (PPA)-induced autism-related behavioral pathology is associated with neurotransmission deficiency caused by disturbances of porosomes. We presume that being posed as physiologically active nano-machines serving cross-membrane transfer of neurotransmitters, porosomes cannot retain normal “viability”, dynamics and functional architecture by PPA-promoted cessation of cell metabolism. Project aims to use amygdala and hippocampal neurons of rat brain, treated in vivo or in vitro with PPA. For in vitro experiments, besides regular 2D cultures, we plan to utilize 3D cultures in order to better mimic tissue/organic physiology and pathology. Advanced photonic microscopy techniques (Optical Tomography, Fluorescent Microscopy, Laser and Super-Resolution Confocal Microscopy) will be utilized in compliance with high-resolution Transmission Electron Microscopy, Scanning Electron Microscopy, Electron Microscopic Tomography and Atomic Force microscopy. Moreover, by visualization of the structural background of PPA-induced metabolic disorder, we will focus on the nucleolus ultrastructure that long been acknowledged as effective criterion assessing the level of r-genes transcription, pre-rRNA processing and pre-ribosomal assembling. The idea is: any cellular activity depends on ribosome production capacity revealed by any given cell.  If we presume that PPA inhibits any metabolic pathway, we shall expect the disturbance of whole metabolism. In turn, incorrectly developing molecular events will cause inhibitory effect upon r-genes that can be registered according nucleolus inactivated phenotype. Obviously, nanoanalytical approach to PPA-affected porosome structure and other fluctuations presumes the neuro-medical basics for future application, for example, in model experiments testing psycho-active drugs, psychiatry etc. Correspondingly, the structure-functional disorders in the nerve cell/tissue architecture revealed by advanced microscopic techniques of micro-, ultra- and nano-analysis are in mainstream of modern neuroscience, meeting increasing requirements of both fundamental neuro-biology and neuromedical tasks.